Detection of fusion genes resulting from chromosome abnormalities in childhood acute lymphoblastic leukemia.
- Author:
Jun HE
1
;
Zi-xing CHEN
;
Yong-quan XUE
;
Jian-qin LI
;
Hai-long HE
;
Yi-ping HUANG
;
Ya-xiang HE
;
Yi-huan CHAI
;
Ling-li ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Child; Child, Preschool; Chromosome Aberrations; Core Binding Factor Alpha 2 Subunit; genetics; metabolism; DNA-Binding Proteins; genetics; metabolism; Flow Cytometry; Homeodomain Proteins; genetics; metabolism; Humans; Immunophenotyping; Infant; Karyotyping; Myeloid-Lymphoid Leukemia Protein; genetics; metabolism; Oncogene Proteins, Fusion; genetics; metabolism; Precursor Cell Lymphoblastic Leukemia-Lymphoma; genetics; metabolism; Proto-Oncogene Proteins; genetics; metabolism; RNA-Binding Protein FUS; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors; genetics; metabolism
- From: Chinese Journal of Medical Genetics 2005;22(5):551-553
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression of the fusion genes resulting from chromosome abnormalities in childhood acute lymphoblastic leukemia(ALL) and its conformity to WHO classification.
METHODSSixty-two children with ALL were investigated. The expression of fusion genes was determined by multiplex reverse transcription-polymerase chain reaction (RT-PCR), karyotyping (R band) and immunophenotyping (by flow cytometry) were also performed.
RESULTSOf the 62 patients, 23(37.1%) were found to carry 13 different fusion genes. The patients with immunophenotype of Pre-B-ALL were found to carry: TEL/AML1(3 cases); E2A/PBX1, E2A/HLF, TLS/ERG, MLL/AF4, MLL/AF9, MLL/AF10, MLL/AFX-MLL/AF6-MLL/ELL, MLL/AF6-MLL/ELL, dupMLL (one case for each); and HOX11 (6 cases). The patients with immunophenotype of Pre-T-ALL were found to carry: TAL1D (4 cases, one is also found to have HOX11 expression); and HOX11 (2 cases). The multiplex RT-PCR in combination with chromosome analysis revealed genetic abnormalities in 69.4%(43/62) of childhood ALL.
CONCLUSIONMultiplex RT-PCR combined with chromosome analysis and immunophenotyping can provide reliable and helpful information for the diagnosis, therapy evaluation and prognosis prediction in childhood ALL, which may also serve as a basis on which to implement the criteria of WHO classification.
