The cloning and expression of apoptosis associated gene ANNEXIN A2 induced by p53 gene.
- Author:
Yun HUANG
1
;
Cheng-hui YAN
;
Song-bin FU
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Annexin A2; genetics; metabolism; Apoptosis; genetics; Base Sequence; Blotting, Northern; Blotting, Western; Cell Line, Tumor; Cloning, Molecular; Gene Expression Regulation, Neoplastic; Humans; Molecular Sequence Data; Reverse Transcriptase Polymerase Chain Reaction; Transfection; Tumor Suppressor Protein p53; genetics; metabolism
- From: Chinese Journal of Medical Genetics 2005;22(6):661-664
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the relationship between p53-dependent apoptosis associated genes and tumor metastasis.
METHODSmRNA differential display (mRNA DD) was adopted for gene cloning after the different metastatic potential lung cancer cell lines were infected by Adv-p53 (a reconstructed adenovirus encoding wild type p53 gene). RT-PCR, Northern blot and Western blot assays were used to confirm the result from mRNA DD.
RESULTSAfter induction by p53 gene, the ANNEXIN A2 gene had differential expression in the cell lines; its level was down regulated in all the cells infected by Adv-p53 gene, especially in the Anip973 cell lines with high metastatic potential. RT-PCR, Northern blot and Western blot assays confirmed the consequence.
CONCLUSIONThe experimental data suggest that the ANNEXIN A2 gene may relate to cellular apoptosis induced by p53 gene. The affirmative relationship between ANNEXIN A2 gene and p53 needs further investigation.
