High resolution DNA typing for HLA-A, B, Cw genes by polymerase chain reaction-sequence specific oligonucleotide probes method in the Shenyang Han population.
- Author:
Shi-xin HAN
1
;
Ying-bin SHANG
;
Jiu-hong LI
;
Long GENG
;
Ning ZHAI
;
Kai CAO
;
Fang-ji SONG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Alleles; China; DNA; analysis; genetics; Female; Gene Frequency; Genotype; HLA-A Antigens; genetics; HLA-B Antigens; genetics; HLA-C Antigens; genetics; Humans; Linkage Disequilibrium; Male; Middle Aged; Oligonucleotide Probes; Polymerase Chain Reaction; methods; Young Adult
- From: Chinese Journal of Medical Genetics 2005;22(6):665-667
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate, at the DNA level, the polymorphism of HLA-A, -B, -Cw genes in the Chinese of Han ethnicity in Shenyang.
METHODSHybridization with polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) was used to determine HLA-A, -B and -Cw genotypes of 108 unrelated healthy individuals from a Chinese Han population. These Hans were born and living in the Shenyang area.
RESULTSThe numbers of alleles identified were 21 for HLA-A, 43 for HLA-B, and 23 for HLA-Cw. All the allele frequency distributions were consistent with the Hardy-Weinberg equilibrium.
CONCLUSIONUsing molecular method, the present authors have analyzed the characteristic of HLA I distribution in a group of indigenous Hans in Shenyang and thus have provided more accurate gene data for use in related researches.
