Effects of NK-1R inhibitor WIN62577 on the migration of airway smooth muscle cells in asthmatic rats with airway remodeling.
- Author:
Bing WEI
1
;
Ya-Li LIU
;
Chao ZHANG
;
Yun-Xiao SHANG
;
Han ZHANG
;
Miao LI
Author Information
- Publication Type:Journal Article
- MeSH: Airway Remodeling; drug effects; Androstenes; pharmacology; Animals; Asthma; pathology; Benzimidazoles; pharmacology; Cell Movement; drug effects; Female; Myocytes, Smooth Muscle; drug effects; physiology; Neurokinin-1 Receptor Antagonists; pharmacology; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Contemporary Pediatrics 2015;17(11):1248-1252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the changes in the migration of airway smooth muscle cells (ASMC) in asthmatic rats with airway remodeling and the effect of NK-1R inhibitor WIN62577 on the migration of ASMC.
METHODSSprague-Dawley rats were randomly assigned into two groups: airway remodeling induced by asthma and normal control. ASMC from rats with asthma and airway remodeling induced by ovalbumin (OVA) inhalation for 8 weeks were primary cultured and purified. Immunofluorescence and real-time PCR were used to measure the expression of NK-1R. With NK-1R inhibitor WIN62577 treatment, the changes in the migration of ASMC were measured by transwell chambers.
RESULTSNK-1R in ASMC was expressed mainly in the cytoplasm and cell membrane in the airway remodeling group, and the mRNA expression of NK-1R was higher than the normal control group (P<0.01). The number of the migrated ASMC in the airway remodeling group was significantly higher than that in the normal control group (P<0.01). Various concentrations (10-11 mol/L, 10-10 mol/L, 10-9 mol/L and 10-8 mol/L) of WIN62577 treatment decreased the number of the migrated ASMC (P<0.05).
CONCLUSIONSNK-1R may affect airway remodeling possibly through promoting the migration ability of ASMC in rats with asthma.
