Effect of Homeobox A13 transfection on epithelial-mesenchymal transition and bone morphogenetic protein-7 expression in kidney tubular epithelial cells.
- Author:
Li PENG
1
;
Qing-Nan HE
;
Xiao-Yan LI
;
Lan-Jun SHUAI
;
Hai-Xia CHEN
;
Yong-Zhen LI
;
Zhu-Wen YI
Author Information
- Publication Type:Journal Article
- MeSH: Bone Morphogenetic Protein 7; genetics; Cells, Cultured; Epithelial Cells; metabolism; Epithelial-Mesenchymal Transition; Homeodomain Proteins; physiology; Humans; Keratins; genetics; Kidney Tubules; metabolism; Transfection; Vimentin; genetics
- From: Chinese Journal of Contemporary Pediatrics 2015;17(12):1342-1347
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the transfection of Homeobox A13 (HOXA13) on epithelial-mesenchymal transition (EMT) and the expression of bone morphogenetic protein-7 (BMP-7) induced by albumin-overload in human kidney tubular epithelial cells (HKCs).
METHODSThe cultured HKCs were treated with 20 mg/mL human serum albumin (HSA) for 48 hours. Protein expression of cytokeratin (CK), vimentin and HOXA13 in the HKCs was assessed by Western blot. Protein expression of CK, vimentin, and BMP-7 was also detected in HKCs transfected with lipofectamine contained HOXA13 DNA.
RESULTSHSA induced EMT in HKCs, presented by decreased CK expression (P<0.01) and increased vimentin expression (P<0.01). The up-regulated expression of HOXA13 transfected by lipofectamine inhibited the level of EMT induced by HSA in HKCs (P<0.05). The decreased rate of BMP-7 protein expression induced by HSA was inhibited by over-expressed HOXA13 in HKCs (P<0.05).
CONCLUSIONSTransfection of HOXA13 in HKCs could inhibit the degree of EMT induced by albumin-overload, possibly by increasing BMP-7 expression.
