Role of long non-coding RNA BC088414 in hypoxic-ischemic injury of neural cells.
- Author:
Feng-Yan ZHAO
1
;
Jun TANG
;
Li ZHANG
;
Shi-Ping LI
;
Yi FENG
;
Hai-Ting LIU
;
Yi QU
;
De-Zhi MU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Caspase 6; genetics; physiology; Cell Hypoxia; Neurons; pathology; PC12 Cells; RNA, Long Noncoding; physiology; RNA, Messenger; analysis; Rats; Receptors, Adrenergic, beta-2; genetics; physiology
- From: Chinese Journal of Contemporary Pediatrics 2015;17(12):1348-1353
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of long non-coding RNA (lncRNA) BC088414 in hypoxic-ischemic injury of neural cells.
METHODSRat adrenal pheochromocytoma (PC12) cells were divided into four groups: normoxic, oxygen glucose deprivation (OGD), siRNA-normoxic (siRNA group) and siRNA-OGD (n=3 each). Cells were incubated in glucose-free and serum-free DMEM medium under the conditions of 37℃ and 1% O2+99% N2/CO2 for 6 hours to establish an in vitro hypoxic-ischemic model. Quantitative real-time PCR was used to measure mRNA expression of lncRNA BC088414, β2-adrenoceptor (Adrb2), and caspase-6 (CASP6). siRNAs were used to inhibit BC088414 expression in PC12 cells. The TUNEL method was used to measure cell apoptosis.
RESULTSThe OGD group had a significantly higher cell apoptotic index than the normoxic group (P<0.01). After inhibition of BC088414 expression, the OGD group had a significantly reduced apoptotic index (P<0.05). The OGD group had significantly higher mRNA expression levels of lncRNA BC088414, Adrb2, and CASP6 compared with the normoxic group (P<0.05). The siRNA -normoxic group had significantly lower mRNA expression levels of Adrb2 and CASP6 than the normoxic group (P<0.05), and the siRNA-OGD group also had significantly lower mRNA expression levels of Adrb2 and CASP6 than the OGD group (P<0.05).
CONCLUSIONSLncRNA BC088414 may promote apoptosis through Adrb2 and CASP6 and aggravate neural cell injury induced by hypoxia-ischemia.
