Roles of PKCα on the biological functions of T cells.
- Author:
Li-Fen YANG
1
;
Hui-Min KONG
;
Xiao-Qing ZHANG
;
Fei YIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; Cytokines; biosynthesis; Lymphocyte Activation; Mice; Protein Kinase C-alpha; physiology; Receptors, Antigen, T-Cell; physiology; T-Lymphocytes; physiology; Th17 Cells; immunology
- From: Chinese Journal of Contemporary Pediatrics 2015;17(12):1354-1359
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the roles of PKCα on the proliferation, apoptosis, differentiation, cytokine production and inducible regulatory T cell (iTreg) induction of T cells.
METHODST cells from WT (PKCα⁺/⁺) or PKCα knockout (PKCα⁻/⁻) mice were isolated and cultured in vitro. T cell proliferation and apoptosis were determined using ³H thymidine incorporation and CSFE/Annexin V staining. Cytokines production (IL-2, IL-4, IFN-γ and IL-17) was detected using ELISA. CD4⁺T cells were isolated and cultured in vitro via Th17 or iTreg biased condition. Flow cytometry was used to detect the cell differentiation.
RESULTSThe production of IL-2 upon TCR stimulation increased, while the contents of IL-4 and IL-17 decreased in the PKCα⁻/⁻ group compared with the PKCα⁺/⁺ group. The differentiation rate of Th17 cells decreased, while the iTreg production increased in the PKCα⁻/⁻ group compared with the PKCα⁺/⁺ group.
CONCLUSIONSPKC-α is proinflammatory.
