The Usefulness of Calretinin Immunohistochemistry for Rectal Suction Biopsy to Diagnose Hirschsprung’s Disease.
10.13029/jkaps.2016.22.2.23
- Author:
Jaeyeop JEONG
1
;
Sang Pyo KIM
;
Eunyoung JUNG
;
Soon Ok CHOI
Author Information
1. Department of Pediatric Surgery, Donsgan Medical Center, Keimyung University School of Medicine, Daegu, Korea. eyjung@dsmc.or.kr
- Publication Type:Original Article
- Keywords:
Hirschsprung disease;
Calbindin 2;
Acetylcholinesterase;
Hematoxylin;
Diagnosis
- MeSH:
Acetylcholinesterase;
Biopsy*;
Calbindin 2*;
Diagnosis;
Ganglion Cysts;
Hematoxylin;
Hirschsprung Disease;
Humans;
Immunohistochemistry*;
Sensitivity and Specificity;
Suction*
- From:Journal of the Korean Association of Pediatric Surgeons
2016;22(2):23-28
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Hirschsprung’s disease (HD) is a congenital intestinal disorder with absence of ganglion cells in the intestinal muscle and submucosa. Diagnosis is based on histopathological study such as H&E, and acetylcholinesterase (AchE) immunohistochemistry. Calretinin immunohistochemistry was introduced as a new diagnostic method against limitations of other staining. The aim of this study is to investigate the usefulness of calretinin immunohistochemistry for the diagnosis of HD compared to H&E and AchE. METHODS: Ten patients with HD and 22 non-HD patients were included in the study. H&E staining, AchE and calretinin immunohistochemistry were performed in all 32 patients. All slides were evaluated by same single pathologist and the diagnostic value was calculated for each H&E stain, AchE immunohistochemical staining, and calretinin immunohistochemical staining. RESULTS: Calretinin method had sensitivity of 100% and specificity of 100% for diagnosis of HD. Its diagnostic accuracy was 100%. AchE staining showed 100% of specificity and 80% of sensitivity. Diagnostic accuracy of H&E staining was 56.3%. CONCLUSION: We concluded that calretinin immunohistochemistry is a very useful and valuable method to diagnosis HD patient.
