Effects of trichostatin A on the interaction between HBx and histone deacetylase protein 1.
- Author:
Ju-qiang HAN
1
;
Qi-nong YE
;
Li-Hua DING
;
Jie-zhi LI
;
Xiao YANG
;
Cui-fen HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Histone Deacetylase 1; metabolism; Humans; Hydroxamic Acids; metabolism; Immunoprecipitation; Plasmids; Protein Interaction Mapping; Trans-Activators; metabolism
- From: Chinese Journal of Hepatology 2008;16(9):657-659
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo study the effects of trichostatin A (TSA) on protein-protein interaction between HBx and histone deacetylase protein 1 (HDAC1).
METHODSBoth HBx and HDAC1 expressing vectors were constructed by the method of routine molecular cloning. The expression of HBx and HDAC1 were observed by Western blot assay. The protein-protein interaction was tested between HBx and HDAC1 by GST pull-down in vitro as well as co-immunoprecipitation in vivo.
RESULTSBoth HBx and HDAC1 expressing vectors were successfully constructed. Protein-protein interaction between HBx and HDAC1 existed both in vitro and in vivo. TSA, an inhibitor of HDAC1, had no effect on the interaction between HBx and HDAC1.
CONCLUSIONSHBx interacts with HDAC1 in vivo and in vitro in a non- TSA dependent way.
