The biological effects of recombinant human bone morphogenetic protein 2 on human periodontal ligament fibroblasts.
- Author:
Xiaohui SI
1
;
Zheng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins; pharmacology; Cell Division; drug effects; Cells, Cultured; Child; Dose-Response Relationship, Drug; Fibroblasts; pathology; Humans; Male; Malocclusion; metabolism; pathology; Osteocalcin; metabolism; Periodontal Ligament; pathology; Recombinant Proteins; pharmacology; Transforming Growth Factor beta
- From: West China Journal of Stomatology 2002;20(1):10-13
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe purpose of this study is to study the biological effects of recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs).
METHODSHuman periodontal ligament fibroblasts were primary cultured and detected the different doses of rhBMP2 on their proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the mineralized nodules.
RESULTSrhBMP2 (0.25-2 mg/ml) had no remarkable effect on the proliferation of HPDLFs. The ALP activity, OC synthesis and formation of the mineralized nodules of HPDLFs were significantly stimulated by 0.5-2 mg/ml rhBMP2.
CONCLUSIONThe effects of rhBMP2 on HPDLFs are dose-dependent. Not only can rhBMP2 stimulate the expression but also promote the maturation of osteoblastic phenotype of HPDLFs.
