Synergistic effects of human tumor necrosis factor-alpha gene transfection and interferon-gamma on the growth of tongue carcinoma cells.
- Author:
Zhennan GAO
1
;
Shengwei LI
;
Jiarang GAO
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; genetics; pathology; Cell Division; drug effects; Cell Survival; drug effects; Dose-Response Relationship, Drug; Drug Synergism; Genetic Vectors; Humans; Interferon-gamma; pharmacology; Plasmids; genetics; Tongue Neoplasms; genetics; pathology; Transfection; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha; genetics
- From: West China Journal of Stomatology 2002;20(1):55-57
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe purpose of this study is to investigate the synergistic effects of human tumor necrosis factor-alpha (hTNF-alpha) transfection and interferon-gamma (IFN-gamma) on the growth of tongue carcinoma cells.
METHODSThe cultured Tca8113 tongue carcinoma cells was divided into 2 groups, one group was transferred with hTNF-alpha gene. Each of the 2 groups was then divided into 5 subgroups, and the subgroups were added IFN-gamma until the final IFN-gamma concentrations respectively were 0, 10, 100, and 1000 U/ml. After culturing for 48 hours, the survival rates of the all groups of cells were assayed by MTT enzymatic labeling technique, and the expression of hTNF-alpha in Tca8113 cells was observed with immunocytochemistry.
RESULTSIFN-gamma did not affect the growth of Tca8113 cells without hTNF-alpha, however, the transfection of hTNF-alpha with the above different concentrations of IFN-gamma synergistically inhibited the growth of Tca8113 cells, the concentrations of IFN-gamma were positively correlated with the inhibition effects (r = 0.733, P < 0.01), the transferred Tca8113 cells displayed remarkable overexpression of hTNF-alpha, compared with the non-transferred.
CONCLUSIONIFN-gamma can enhance the inhibition of hTNF-alpha transfection on the tongue carcinoma cells.