Effects of epidermal growth factors on the proliferation and metabolism of A/J mouse embryonic palatal cells.
- Author:
Jinhu SUN
1
;
Bing SHI
;
Dazhang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Division; drug effects; Cells, Cultured; DNA; analysis; Dinoprostone; biosynthesis; Dose-Response Relationship, Drug; Embryo, Mammalian; Epidermal Growth Factor; pharmacology; Mice; Palate; cytology
- From: West China Journal of Stomatology 2002;20(3):161-163
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe purpose of this study was to investigate the effects of epidermal growth factors (EGFs) with different concentration on the OD, DNA, protein, and PGE2 of A/J mouse embryonic palatal shelves cells (A/J MEPC) isolated from embryonic palatal shelves.
METHODSThe mouse embryonic palatal shelves cells were grown in different 39 pores (or bottles) with 9 gradient concentrations of EGF (0.005, 0.010, 0.050, 0.100, 0.500, 1.000, 5.000, 10.000, 50.000 ng/ml), and four pores were prepared for the same concentration, then the OD, DNA, protein and PGE2 of A/J MEPC were measured after 1 day, 3 days and 5 days.
RESULTSEGFs stimulated DNA and PGE2 synthesis of A/J MEPC, and augmented proliferation index (PIX). Their effects were very obvious in promoting the proliferation of A/J MEPC, when the concentration was 10.000 ng/ml.
CONCLUSIONEGF may be important in regulating proliferation and metabolism of embryonic palatal shelves cells.
