A study on screening effective immunization route of anticaries DNA vaccine pcDNA3-gtfB.
- Author:
Jinbo YANG
1
;
Tianjia LIU
;
Heng ZHUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Bacterial; biosynthesis; Bacterial Vaccines; administration & dosage; immunology; Cloning, Molecular; Dental Caries; prevention & control; Glucosyltransferases; genetics; immunology; Immunization; Immunoglobulin A, Secretory; analysis; Immunoglobulin G; blood; Male; Plasmids; genetics; immunology; Random Allocation; Rats; Rats, Wistar; Recombination, Genetic; Saliva; immunology; Streptococcus mutans; genetics; immunology; Vaccines, DNA; administration & dosage; immunology
- From: West China Journal of Stomatology 2002;20(5):374-376
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEGlucosyltransferase-B (GTF-B) of Streptococcus mutans has been implicated as a principal virulent factor in the development of dental caries. The objective was to use recombined plasmid pcDNA-gtfB expressing multiple antigen of glucosyltransferase-B as gene vaccine to immunize rats through different route, and to investigate the immunization effects of immunization routes.
METHODSA total of 18 Wistar rats were divided into 3 groups, including the quadriceps injection group, the intransal irrigation group and the submandibular gland-targeted injection group. The serum IgG and salivary IgA were assayed by using ELISA after pcDNA3-gtfB immunization. The serum IgG and salivary IgA in different groups were compared using statistical one-way ANOVA.
RESULTSCompared these 3 groups, the serum IgG in the quadriceps injection group was much higher than those of other two groups (P < 0.01), while the salivary IgA of the submandibular gland-targeted injection was much higher than those of other two groups (P < 0.01).
CONCLUSIONIt is indicated pcDNA3-gtfB is good candidate for anticarious gene vaccine, and submandibular gland-targeted injection is an effective immunization route for stimulating salivary IgA.
