An experimental study on circadian rhythm of the proliferative index of mandibular osteoblast in goats.
- Author:
Xiaohui ZHENG
1
;
Weidong TIAN
;
Jie LONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Division; Cells, Cultured; Circadian Rhythm; Flow Cytometry; Goats; Mandible; cytology; Osteoblasts; cytology; Random Allocation
- From: West China Journal of Stomatology 2003;21(1):10-27
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe aim of this study was to investigate the circadian rhythm of the proliferative index of mandibular osteoblasts of goats in a normal situation.
METHODSTwenty four healthy goats were selected and randomly divided into six groups. They were killed, and a piece of bone tissue with 2 cm x 2 cm x 0.5 cm was obtained from right mandibular mental foramen region at 0:00, 4:00, 8:00, 12:00, 16:00 and 20:00 time points, after they had been fed for one week. The primary cell culture method was used to cultivate osteoblasts from the bone. The method of anti-5-bromodeoxyuridine monoclonal antibody and the flow cytometric analysis were used to detect the proliferative index of the mandibular osteoblasts at different time. The data were analyzed using the method of single cosine rhythmometry.
RESULTSThe proliferative indexes of the mandibular osteoblasts of goats were higher at the nighttime and lower in the daytime. The undulation of the proliferative indexes displayed a significant circadian rhythm (P < 0.01). The peak value was obtained at about 19:44, and the lowest value at about 7:44.
CONCLUSIONThe proliferative indexes of the mandibular osteoblasts of goats are not stable within 24 hours in a normal situation, but with significant circadian rhythm. There are different proliferative indexes at different time points.