An experimental study on gene transfection of human interleukin-1 receptor antagonist gene into chondrocytes of temporomandibular joint.
- Author:
Yisong LI
1
;
Weidong TIAN
;
Dong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chondrocytes; cytology; Enzyme-Linked Immunosorbent Assay; Fetus; Phosphatidylethanolamines; Receptors, Interleukin-1; antagonists & inhibitors; biosynthesis; genetics; Recombinant Proteins; biosynthesis; genetics; Temporomandibular Joint; cytology; Transfection
- From: West China Journal of Stomatology 2003;21(1):19-21
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe purpose of this study was to investigate methods and expression of the recombinant human interleukin-1 receptor antagonist gene transfected into chondrocytes of temporomandibular joints (TMJ).
METHODSChondrocytes derived from the TMJ of 5-7 months old human fetus were transfected by recombinant human interleukin-1 receptor antagonist gene via cationic liposome (Lipefectine) as a medium. The expressing level of hIL-1ra protein was detected using immunocytochemistry and enzyme-linked immunosorbent assay (ELISA).
RESULTSThe positive results of the immunocytochemistry were found. The positive expression was detected in the cell plasma and the cell culture supernatant, and there was significant difference between the cells with and without gene transfection (P < 0.01).
CONCLUSIONThis feasible method provides experimented evidence for the future gene therapy of temporomandibular joint osteoarthrosis.
