Human anti-HCMV neutralizing Fab antibody generated by phage display library.

Author: Tao DUAN ¹ ; Mi-fang LIANG ; Shu-yan GU
Author Information

1. Virus Emergency Biotech Center, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Publication Type:Journal Article
MeSH: Antibodies, Viral; genetics; immunology; Cytomegalovirus; genetics; immunology; Cytomegalovirus Infections; immunology; virology; Escherichia coli; genetics; metabolism; Gene Expression; Humans; Immunoglobulin Fab Fragments; genetics; immunology; Neutralization Tests; Peptide Library
From: Chinese Journal of Experimental and Clinical Virology 2003;17(3):247-250
CountryChina
Language:Chinese
Abstract:
BACKGROUNDCloning recombinant human Fab fragment against HCMV for the purpose of prophylaxis and control of HCMV infection.
METHODSThe authors constructed a HCMV phage display library with 2 x 10(6) clones, then used purified HCMV viral lysates to pan the library, then screened by ELISA.
RESULTSThree clones showed positive responses in ELISA, they also showed high specificity in IFA, two of them could neutralize HCMV in neutralizing assays.
CONCLUSIONThe specific binding of Fab antibodies to HCMV was demonstrated by ELISA, IFA and neutralizing activities. These results provide us the basis for further research of neutralizing recombinant human whole IgG molecule.