Inclusion bodies of human cytomegalovirus are composed of the DNA and immediately early and early antigens of the virus.
- Author:
Qing-guo YAN
1
;
Gao-sheng HUANG
;
Ying GUO
;
Zhe WANG
;
Ji-liang FENG
;
Guo-rong YANG
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Viral; analysis; immunology; Cytomegalovirus; genetics; immunology; Cytomegalovirus Infections; diagnosis; immunology; virology; DNA, Viral; analysis; genetics; Humans; Immunohistochemistry; Inclusion Bodies; chemistry; immunology; virology; Microdissection; Salivary Glands; chemistry; immunology; virology
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(3):262-265
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo study the composition and significance of the inclusion bodies of human cytomegalovirus (HCMV).
METHODSMicrodissection of inclusion bodies, PCR and Southern blot were adopted to detect DNA, and immunohistochemistry method and catalyzed signal amplification (CSA) were used to detect the different antigens of HCMV.
RESULTSThe inclusion bodies of HCMV were separated from the tissue section of human salivary gland. The fragments amplified by PCR from these dissected inclusion bodies were confirmed to be the DNA of HCMV. With the immunohistochemical method CSA, the immediately early and early antigens of HCMV were detected with monoclonal antibodies DDG9/CCH2, while matrix protein AAC10 was negative in the inclusion bodies.
CONCLUSIONThe ingredient of inclusion bodies of HCMV included the DNA and the antigens expressed in specific stage of the virus.
