Capturing-ELISA for the detection of anti-HCMV-IgM antibody in serum.
- Author:
Xin-ping ZHANG
1
;
Pei LI
;
Hong-jing LIU
;
Bao-gen LIU
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Viral; blood; Cytomegalovirus; immunology; Cytomegalovirus Infections; immunology; virology; Enzyme-Linked Immunosorbent Assay; methods; Humans; Immunoglobulin M; blood
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(3):285-286
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo develop a capturing-ELISA for the detection of anti-HCMV-IgM antibody in serum.
METHODSThe anti-HCMV-IgM antibody was detected in 68 patients with HCMV infection by the capturing-ELISA, and the results were compared with those of indirect ELISA.
RESULTSThe specificity and sensitivity of the capturing-ELISA were shown to be significantly higher than those of indirect ELISA, and its results were not affected by RF factor.
CONCLUSIONThe capturing ELISA is specific, sensitive, convenient and reliable method which may be feasible for clinical use.
