Full-length sequence of hepatitis B virus isolated from high incidence hepatocellular carcinoma area-Longan county.
- Author:
Xian-min GE
1
;
Zhong-liao FANG
;
Dan-ya LI
;
Lian-san ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Amino Acid Sequence; Base Sequence; Carcinoma, Hepatocellular; epidemiology; Carrier State; virology; China; epidemiology; Genotype; Hepatitis B; virology; Hepatitis B virus; genetics; Humans; Incidence; Liver Neoplasms; epidemiology; Male; Molecular Sequence Data; Open Reading Frames; genetics; Point Mutation; Sequence Analysis, DNA; Sequence Homology
- From: Chinese Journal of Experimental and Clinical Virology 2004;18(3):272-276
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo understand full-length sequence of HBV isolated from high incidence hepatocellular carcinoma area-Longan county, Guangxi.
METHODSThe nested polymerase chain reaction (nPCR) was used for amplifying the whole HBV DNA in sera of asymptomatic carriers. The products were sequenced by clone sequencing and homological analysis.
RESULTSThis isolate contained 3 215 bases. The genotype was C and the serotype was adw. There were 40 point mutations in polymerase gene which made 11 amino acids change. There were 11,2 and 3 point mutations in PreS1, PreS2 and S gene respectively which made 3,1,1, amino acids change. Six point mutations including the double mutations (nt 1762 A to T, 1764 G to A) were found in X gene leading to 4 amino acids change. There were 13 point mutations in C gene which made 2 amino acids change. No mutation was found in a determinant and Pre C. The isolate was quite close to the isolate from Vietnamese in evolution while far from the genotype C isolates from Shanghai, Beijing and Tibet.
CONCLUSIONNo special sequence was found in the isolate from high incidence hepatocellular carcinoma area, Longan county, Guangxi.
