Application of fluorescent real-time reverse transcriptase-polymerase chain reaction in detecting influenza viruses.
- Author:
Xiao-wen CHENG
1
;
Li ZHOU
;
Jin ZHAO
;
Shi-song FANG
;
Lei YU
;
Bao-ying YE
;
Jian-fan HE
;
Xing LU
;
Zai-qing ZHANG
;
Hong YANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Culture Techniques; Humans; Influenza A Virus, H3N2 Subtype; isolation & purification; Influenza A virus; isolation & purification; Influenza, Human; virology; Reverse Transcriptase Polymerase Chain Reaction; methods; Sensitivity and Specificity; Severe Acute Respiratory Syndrome; virology
- From: Chinese Journal of Experimental and Clinical Virology 2004;18(3):289-290
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo apply fluorescent real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in detecting influenza viruses.
METHODSA total of 207 oral swab samples were obtained in 16 collections from SARS patients and suspected influenza outbreak cases. They were subjected to influenza virus detection by fluorescent real-time RT-PCR, MDCK cell culture, and hemagglutinin inhibition assay.
RESULTSOut of 207 samples, 79 (38.16%) were positive for influenza viruses when tested by fluorescent real-time PCR, and 62 (29.95%) positive when tested by MDCK cell culture. There was a statistically significant difference between them (chi square=8.64, P less than 0.005). From 104 cases in 9 collections dual serum samples were obtainable. When tested with hemagglutinin inhibition assay, 64 cases (61.54%) showed a 4-fold increase against H3N2 antigen.
CONCLUSIONThis study showed that fluorescent real-time PCR is a reliable, sensitive, and fast method for detecting influenza viruses.
