Coxsackie virus B types were discriminated by RT-PCR.
- Author:
Zhen-yong LI
1
;
Zhi-tao LI
;
Yan-ming FENG
;
Da-xiao SHAO
;
Da-peng ZHAO
;
Tian-xing CUI
;
Guo-cui YANG
;
Ling-bo QU
;
Yu-fen ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: DNA Primers; Enterovirus B, Human; classification; genetics; isolation & purification; Enterovirus Infections; diagnosis; virology; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin M; blood; Reverse Transcriptase Polymerase Chain Reaction; methods
- From: Chinese Journal of Experimental and Clinical Virology 2004;18(3):291-293
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for detection of coxsackie B virus type 1-6 by RT-PCR.
METHODSA pair of primers were designed to amplify all types of coxsackie B virus 1-6 efficiently. The PCR product was hybridized in micro-wells in which 6 type specific oligonucleotide probes had been coated respectively, colorimetric detection was performed to discriminate the types of coxsackie B virus.
RESULTSThis method was shown to be concordant with the IgM ELISA, 71.7% of anti-coxsackie B positive cases could be detected by RT-PCR.
CONCLUSIONThe RT-PCR method can type coxsackie B virus efficiently and provides a tool for clinical diagnosis and epidemiological investigation.
