Dynamic expression of matrix metalloproteinase-2, membrane type-matrix metalloproteinase-2 in experimental hepatic fibrosis and its reversal in rat.
- Author:
Zhi-hai ZHAO
1
;
Shao-jie XIN
;
Jing-min ZHAO
;
Song-shan WANG
;
Ping LIU
;
Tie-yong YIN
;
Guang-de ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carbon Tetrachloride Poisoning; Gene Expression Regulation, Enzymologic; Hepatocytes; enzymology; Liver; enzymology; pathology; Liver Cirrhosis, Experimental; enzymology; etiology; pathology; Male; Matrix Metalloproteinase 2; biosynthesis; genetics; Matrix Metalloproteinases; biosynthesis; genetics; Matrix Metalloproteinases, Membrane-Associated; Mesenchymal Stromal Cells; enzymology; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Wistar
- From: Chinese Journal of Experimental and Clinical Virology 2004;18(4):328-331
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression dynamics and significance of matrix metalloproteinase-2 (MMP-2) membrane type-matrix metalloproteinase-2 (MT-MMP-2) in hepatic fibrosis and its reversal counterpart.
METHODSAn experimental CCl4 induced hepatic fibrosis rat model was established by intraperitoneal administration of carbon tetrachloride for 2, 4, 6, 8, 10 weeks, and normal rats were used as a control group. The immunohistochemical methods and in situ hybridization were used to detect MMP-2,MT-MMP-2 mRNA and related antigens in the liver.
RESULTSMMP-2,MT-MMP-2 mRNA and related antigens were expressed in mesenchymal cells and parts of hepatocytes besides active pathological changes, especially in the fibrous septum and portal area. Expression of MMP-2,MT-MMP-2 mRNA and related antigens were increased in hepatic fibrosis and decreased gradually in its reversal counterpart.
CONCLUSIONThis study suggested that mesenchymal cells are the main cellular origins of MMPs. The levels of MMP-2 and MT-MMP-2 antigens and gene expression were closely related to hepatic fibrosis. MMP-2 and MT-MMP-2 may play important roles in hepatic fibrosis and its reversal counterpart.
