Micro-droplet characterization and its application for amino acid detection in droplet microfluidic system.
- Author:
Huiling YUAN
;
Libing DONG
;
Ran TU
;
Wenbin DU
;
Shiru JI
;
Qinhong WANG
- Publication Type:Journal Article
- MeSH:
Amino Acids;
isolation & purification;
Microfluidic Analytical Techniques;
Microfluidics;
instrumentation
- From:
Chinese Journal of Biotechnology
2014;30(1):139-146
- CountryChina
- Language:Chinese
-
Abstract:
Recently, the droplet microfluidic system attracts interests due to its high throughput and low cost to detect and screen. The picoliter micro-droplets from droplet microfluidics are uniform with respect to the size and shape, and could be used as monodispensed micro-reactors for encapsulation and detection of single cell or its metabolites. Therefore, it is indispensable to characterize micro-droplet and its application from droplet microfluidic system. We first constructed the custom-designed droplet microfluidic system for generating micro-droplets, and then used the micro-droplets to encapsulate important amino acids such as glutamic acid, phenylalanine, tryptophan or tyrosine to test the droplets' properties, including the stability, diffusivity and bio-compatibility for investigating its application for amino acid detection and sorting. The custom-designed droplet microfluidic system could generate the uniformed micro-droplets with a controllable size between 20 to 50 microm. The micro-droplets could be stable for more than 20 h without cross-contamination or fusion each other. The throughput of detection and sorting of the system is about 600 micro-droplets per minute. This study provides a high-throughput platform for the analysis and screening of amino acid-producing microorganisms.
