Effects of chemically modified sugarcane bagasse on butanol production by immobilized Clostridium acetobutylicum XY16.
- Author:
Xiangping KONG
;
Aiyong HE
;
Jianan CHEN
;
Wufang CHEN
;
Chunyan YIN
;
Pan CHEN
;
Hao WU
;
Min JIANG
- Publication Type:Journal Article
- MeSH:
Batch Cell Culture Techniques;
Bioreactors;
Butanols;
metabolism;
Cells, Immobilized;
Cellulose;
metabolism;
Clostridium acetobutylicum;
metabolism;
Fermentation;
Saccharum;
chemistry
- From:
Chinese Journal of Biotechnology
2014;30(2):305-309
- CountryChina
- Language:Chinese
-
Abstract:
Sugarcane bagasse modified by polyethylenimine (PEI) and glutaraldehyde (GA) was used as a carrier to immobilize Clostridium acetobutylicum XY16 in the process of butanol production. The effects of chemically modified sugarcane bagasse on batch and repeat-batch fermentations were investigated. Batch fermentation was conducted with an addition of 10 g/L modified sugarcane bagasse and 60 g/L glucose, resulting in a high solvent concentration of 21.67 g/L and productivity of 0.60 g/(L x h) with the treatment of 4 g/L PEI and 1 g/L GA. Compared to the fermentations by free cells and immobilized cells on unmodified sugarcane bagasse, the productivity increased 130.8% and 66.7%, respectively. The fibrous-bed bioreactor also maintained a stable butanol production during repeat-batch fermentations, achieving a maximum productivity of 0.83 g/(L x h) with a high yield of 0.42 g/g.
