Production of L(+)-tartaric acid by immobilized Rhizobium strain BK-20.
- Author:
Xiang LAN
;
Wenna BAO
;
Haifeng PAN
;
Zhipeng XIE
;
Jianguo ZHANG
- Publication Type:Journal Article
- MeSH:
Alginates;
chemistry;
Cells, Immobilized;
Glucuronic Acid;
chemistry;
Hexuronic Acids;
chemistry;
Hydrolases;
metabolism;
Rhizobium;
enzymology;
metabolism;
Tartrates;
metabolism
- From:
Chinese Journal of Biotechnology
2014;30(2):315-319
- CountryChina
- Language:Chinese
-
Abstract:
The cis-epoxysuccinate hydrolase (CESH) from Rhizobium strain BK-20 is the key enzyme for L(+)-tartaric acid production. To establish a highly efficient and stable production process, we first optimized the enzyme production from Rhizobium strain BK-20, and then developed an immobilized cell-culture process for sustained production of L(+)-tartaric acid. The enzyme activity of free cells reached (3 498.0 +/- 142.6) U/g, and increased by 643% after optimization. The enzyme activity of immobilized cells reached (2 817.2 +/- 226.7) U/g, under the optimal condition with sodium alginate as carrier, cell concentration at 10% (W/V) and gel concentration at 1.5% (W/V). The immobilized cells preserved high enzyme activity and normal structure after 10 repeated batches. The conversion rate of the substrate was more than 98%, indicating its excellent production stability.
