Preparation and immunogenicity of silk fibroin/chitosan microspheres for DNA vaccine delivery against infectious bursal disease virus.
- Author:
Yan LIU
;
Zhiqiang LV
;
Cun ZHANG
;
Xingrong ZHU
;
Tuanyuan SHI
;
Shi ZHONG
;
Zhiqi MENG
- Publication Type:Journal Article
- MeSH:
Adjuvants, Immunologic;
chemistry;
Animals;
Birnaviridae Infections;
prevention & control;
veterinary;
Chickens;
Chitosan;
chemistry;
Fibroins;
chemistry;
Infectious bursal disease virus;
Microspheres;
Plasmids;
Poultry Diseases;
prevention & control;
Vaccines, DNA;
chemistry;
Viral Vaccines;
chemistry
- From:
Chinese Journal of Biotechnology
2014;30(3):393-403
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the immunities of biodegradable microsphere as a release delivery system for DNA vaccine against Infectious Bursal Disease Virus, in our study, silk fibroin/chitosan microsphere adjuvant was prepared with a precipitation/coacervation method. Both glutaraldehyde and Na2SO4 solution were used in cross-linking. No immune chicken were intramuscularly inoculated at 14 day-old and boosted 2 weeks later. The results show that glutaraldehyde destroyed the DNA activity of the vaccine whereas Na2SO4 solution did not. Factors of the chitosan concentration 0.5% (pH 5.0), silk fibroin concentration 0.6%, plasmid DNA (500 microg/mL) dissolved in 2% Na2SO4 solution were optimized to produce microsphere, with a loading capacity of 89.14%. The average particle size of SF-CS/pCI-VP2/4/3 microsphere is 1.98 microm, and it can protect the loading DNA vaccine from DNase I digestion. Data from anti IBDV ELISA antibodies in the serum show that immunization activity of the microsphere groups were generally higher than plasmid vaccine group (P < 0.05), and the SF/CS compound microspheres group was better than that of sole CS microsphere group. The developed SF/CS microspheres are a very promising vaccine delivery system.
