Optimization of manganese peroxidase production from Schizophyllum sp. F17 in solid-state fermentation of agro-industrial residues.
- Author:
Yue ZHOU
;
Bing YANG
;
Yang YANG
;
Rong JIA
- Publication Type:Journal Article
- MeSH:
Culture Media;
Fermentation;
Industrial Microbiology;
methods;
Oryza;
Peroxidases;
biosynthesis;
Schizophyllum;
enzymology;
Wood
- From:
Chinese Journal of Biotechnology
2014;30(3):524-528
- CountryChina
- Language:English
-
Abstract:
Manganese peroxidase (MnP), a crucial enzyme in lignin degradation, has wide potential applications in environmental protection. However, large-scale industrial application of this enzyme is limited due to several factors primarily related to cost and availability. Special attention has been paid to the production of MnP from inexpensive sources, such as lignocellulosic residues, using solid-state fermentation (SSF) systems. In the present study, a suitable SSF medium for the production of MnP by Schizophyllum sp. F17 from agro-industrial residues has been optimized. The mixed solid medium, comprising pine sawdust, rice straw, and soybean powder at a ratio of 0.52:0.15:0.33, conferred a maximum enzyme activity of 11.18 U/g on the sixth day of SSF. The results show that the use of wastes such as pine sawdust and rice straw makes the enzyme production more economical as well as helps solve environmental problems.
