Comparison of two urinary protein preparation methods: nitrocellulose membrane preservation and acetone precipitation.
- Author:
Xiaorong WANG
;
Xundou LI
;
Lulu JIA
;
Youhe GAO
- Publication Type:Journal Article
- MeSH:
Acetone;
Chromatography, Liquid;
Collodion;
Humans;
Mass Spectrometry;
Proteins;
isolation & purification;
Reproducibility of Results;
Tandem Mass Spectrometry;
Urine;
chemistry
- From:
Chinese Journal of Biotechnology
2014;30(6):982-989
- CountryChina
- Language:Chinese
-
Abstract:
Nitrocellulose membrane based urinary protein preservation method is simple, fast and economic, but its advantage over the traditionally used acetone precipitation method is still unclear. In this work, we prepared urinary proteins by the two methods by LC-MS/MS. Then we used protein spectra counts to assess the reproducibility of the two methods. Proteins identified by the two methods were almost the same in number, spectral count distribution and distribution of coefficients of variation value. In conclusion, nitrocellulose membrane method is generally the same as acetone precipitation method. It can be used for large scale preservation of clinical urine samples.
