Laser microdissection and mass spectrometry based proteomics in the diagnosis of kidney diseases.
- Author:
Ying SUN
;
Mingxi LI
;
Yubing WEN
;
Xuemei LI
;
Jian SUN
;
Wei SUN
- Publication Type:Journal Article
- MeSH:
Formaldehyde;
Humans;
Kidney;
pathology;
Kidney Diseases;
diagnosis;
Laser Capture Microdissection;
Mass Spectrometry;
Proteomics;
Tissue Fixation
- From:
Chinese Journal of Biotechnology
2014;30(7):1134-1140
- CountryChina
- Language:Chinese
-
Abstract:
In recent years, laser microdissection followed by mass spectrometry (LMD/MS) has been successfully applied to the proteomic studies of formalin-fixed paraffin-embedded (FFPE) renal tissues. This new technique improves the diagnosis of kidney diseases and has a better potential for future clinical application. The review focuses on the use of this methodology for exploring the mechanisms, diagnosis and classification of kidney diseases including renal amyloidosis and membrane proliferative glomerulonephritis.
