Determination of baicalin in Shuanghuanglian oral liquid on composite mercury film electrode based on chemical modified.
- Author:
Qingyun SONG
1
;
Yongjun MA
;
Xiufang HOU
Author Information
- Publication Type:Journal Article
- MeSH: Adsorption; Dosage Forms; Drugs, Chinese Herbal; analysis; Electrochemistry; instrumentation; methods; Electrodes; Flavonoids; analysis; Mercury; chemistry; Scutellaria; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(18):2399-2401
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA novel Eu-PB composite mercury film electrode (GC/Eu-PB/MFE) based on chemical modified was developed for the determination of baicalin and the investigation of its the electrochemical behavior.
METHODCyclic voltammetry and differential pulse voltammetry were used.
RESULTThe adsorption characteristic of baicalin was observed in Britton-Robinson buffer solution (pH 6.0) at GC/Eu-PB/MFE. The reduction peak of baicalin was observed at -1.22 V (vs SCE) and peak current was linear to the baicalin from 8.0 x 10(-9) to 1.0 x 10(-6) mol x L(-1) (r = 0.9992, n=8). The limit of detection was 1.2 x 10(-9) mol x L(-1) (S/N = 3). The average recovery was 99.83%, with RSD of 2.3% (n=8). The baicalin in Shuanghuanglian oral can be directly determined without pretreatment.
CONCLUSIONThe GC/Eu-PB/MFE was used to determine baicalin in Shuanghuanglian oral with good repeatability and high sensitivity. The method can be used for the quality control of Chinese traditional medicines containing Scutellaria baicalensis.
