Variety regulation of aloe-emodin-3-CH2-O-beta-D-glucopyranoside and emodin-8-O-beta-D-glucopyranoside in five processed pieces from Rheum palmatum.
- Author:
Guofang TIAN
1
;
Cun ZHANG
;
Li LI
;
Yongqing XIAO
;
Dongdong CHEN
;
Yun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chemistry, Pharmaceutical; methods; Drugs, Chinese Herbal; analysis; Glucosides; analysis; Rheum; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(18):2437-2439
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the contents of two another anthraquinone-O-glucosides in five different processed products from Rheum palmatum.
METHODAloe-emodin-3-CH2-O-beta-D-glucopyranoside and emodin-8-O-beta-D-glucopyranoside were determined simultaneously by HPLC on an Agilent TC-C18 (2) column at 35 degrees C with the tetrahydrofuran-1% glacial acetic acid (25:75). The detection wavelength was set at 410 nm and the flow rate was 0.9 mL x min(-1).
RESULTThe calibration curves for two components were linear within the range of 0.0090-0.2704 microg (r = 0.9996), 0.0396-1.188 microg (r = 0.9996), and the average recoveries were 97.85%, 97.19% respectively.
CONCLUSIONThe results showed the remarkable variation regulations among the five different processed pieces. The contents of the crude pieces and the pieces roasted vinegar were almost the same, while the pieces roasted with millet wine were a little higher than that of the crude pieces, and the contents at the two components in prepared pieces and charring pieces evidently reduced.
