Effect of brazilein on energy metabolism of cerebral ischemia-reperfusion in mice.
- Author:
Huiying LI
1
;
Yunyun CHEN
;
Fan LEI
;
Jun HU
;
Jiaqi LAN
;
Yushuang CHAI
;
Dongming XING
;
Lijun DU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzopyrans; administration & dosage; Brain Ischemia; drug therapy; genetics; metabolism; Disease Models, Animal; Energy Metabolism; drug effects; Gene Expression; drug effects; Humans; Indenes; administration & dosage; Male; Mice; Mice, Inbred ICR; Monocarboxylic Acid Transporters; genetics; metabolism; Random Allocation; Reperfusion Injury; drug therapy; genetics; metabolism; Symporters; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2010;35(18):2444-2448
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate brazilein's role in energy metabolism of cerebral ischemia-reperfusion in mice.
METHODFourty mice were randomly divided into the sham group, ischemia group, brazilein 5 mg x kg(-1) group and brazilein 10 mg x kg(-1) group, each with ten cases. Cerebral ischemia model was the built. Mice were injected with brazilein three days before the operation, then they were killed. Cerebrum homogenate was prepared for the detecting of ATP, ADP, AMP and lactic acid by HPLC, expressions of MCT1 and MCT2 in mRNA level by RT-PCR.
RESULTThe lactic acid in cerebrum increased sharply 20 minutes after cerebral ischemia and decreased 1 hour after reperfusion, then returned to the normal level 24 hours after reperfusion. The charge of energy decreased significantly at the beginning of the ischemia-reperfusion, and the charge restored 1 hour after reperfusion though it was still much lower than the normal level at the time point of 24 hours. Moreover, MCT1 and MCT2 upregulated accompanied with the increase of lactate, MCT2 mRNA enhanced in brazilein 5 mg x kg(-1) group (P < 0.05) while both the two factors increased in brazilein 10 mg x kg(-1) group (P < 0.01).
CONCLUSIONBrazilein might protect neurons by changing the charge of energy.
