Mechanism of protective effect of pretreatment of paeoniflorin on injured cortical neurons with corticosterone.
- Author:
Yang LIU
1
;
Guangzhi CUI
;
Yanjun ZHANG
;
Xiumei GAO
;
Yan GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzoates; pharmacology; Brain-Derived Neurotrophic Factor; genetics; metabolism; Bridged-Ring Compounds; pharmacology; Caspase 3; genetics; metabolism; Cells, Cultured; Cerebral Cortex; cytology; drug effects; injuries; metabolism; Corticosterone; adverse effects; Female; Gene Expression; drug effects; Glucosides; pharmacology; Male; Monoterpenes; Neurons; drug effects; metabolism; Neuroprotective Agents; pharmacology; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2010;35(18):2457-2459
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEUsing the method of reverse transcription-polymerase chain reaction (RT-PCR) to detect the related gene expression,with a view to clarify the principal mechanism that the neuroprotection of paeoniflorin (PF).
METHODAt the seventh day, after the pretreatment of PF (0.5, 2, 10 micromol x L(-1)), with 30 min primary cultures of neurons from the cerebral cortex of 16 days old embryo rats were injured with corticosterone (200 micromol x L(-1)). After 48 h, the methods of RT-PCR was used to detect the expression of Bcl-2, Bax, Caspase-3, BDNF mRNA.
RESULTCompared with model group, the dose of PF(2, 10 micromol x L(-1)) not only can degrade the expression of Bax, Caspase-3 mRNA, but also can raise the expression of Bcl-2, BDNF mRNA notablely (P < 0.05).
CONCLUSIONThe data indicated that the principal mechanism of prophylactic neuroprotective effect of PF on corticosterone-induced neurons damages maybe due to PF can raise the expression of apoptosis-related genes Bel-2, Bax, Caspase-3 mRNA, and also ascribe to PF can raise the expression of neurotrophic factor.
