Studies on reversing MDR of K562/A02 by ramification of curcumin hydrolyzed.
- Author:
Yanfen HUANG
1
;
Gaixia DONG
;
Xingqiu HONG
;
Hui CHAI
;
Xiaofeng YUAN
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; genetics; metabolism; Curcumin; chemistry; pharmacology; Drug Resistance, Multiple; drug effects; Drug Resistance, Neoplasm; drug effects; Drugs, Chinese Herbal; chemistry; pharmacology; Gene Expression; drug effects; Humans; K562 Cells; Neoplasms; drug therapy; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2010;35(18):2460-2463
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the reversal effects of ramification of curcumin hydrolyzed on multidrug-resistant cell line K562/A02, and explore its reversal mechanism.
METHODAfter treatment with ramification of curcumin hydrolyzed, the sensitivity of K562/A02 cells to usuall chemotherapeutic drugs were determined by MTT. The expression of P-gp in K562/A02 and K562 cells was detected by immunohistochemical method. Intracellular mean fluorescence intensity (MFI) of DNR in K562 and K562/A02 cells was detected by Flow Cytometry.
RESULTAfter treatment with the ramification of curcumin, hydrolyzed (2.5 mg x L(-1)) IC50, of usuall chemotherapeutic drugs to K562/A02 decreased. The sensitivity of K562/A02 cells increased. The expression of the P-gp in K562/A02 cells decreased (P < 0.05); MFI of the DNR in K562/A02 cells more significantly increased (P < 0.05). The expression of mdrl-mRNA decreased.
CONCLUSIONThe ramification of curcumin hydrolyzed have effects on the reversal multidrug-resistant of K562/A02 cells in vitro. It could enhance the sensitivity of K562/A02 cells to chemotherapeutic drugs and the mechanism might be associated with inhibiting P-gp-mediated drug efflux and increasing of intracellular concentration of chemotherapeutic drugs.
