DNA barcoding in medicinal plants Caprifoliaceae.

Author: Zhen LIU ¹ ; Keli CHEN ; Kun LUO ; Honglin PAN ; Shilin CHEN
Author Information

1. Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei College of Traditional Chinese Medicine, Wuhan 430065, China.
Publication Type:Journal Article
MeSH: Automatic Data Processing; methods; Base Sequence; Caprifoliaceae; genetics; DNA; analysis; DNA, Plant; analysis; Identification (Psychology); Plants, Medicinal; genetics; Polymerase Chain Reaction; methods; Species Specificity
From: China Journal of Chinese Materia Medica 2010;35(19):2527-2532
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo determine the candidate sequences which can be used as DNA barcode to identify species in Caprifoliaceae family by screening out from four different DNA fragments sequences.
METHODPCR amplification, sequencing efficiency, differential intra- and interspecific divergences, the DNA barcoding gap and identification efficiency were used to evaluate these loci.
RESULTThe ITS2 was used as a candidate sequence of DNA barcode to identify the species in Caprifoliaceae family, whose rate of success in identification in genera level was 100% and in species 96.6%, and psbA-trnH as a complementary barcode to ITS2 for Caprifoliaceae.