Quantitative analysis of five components in the rhizome of Angelica polymorpha by RP-HPLC under different UV wavelengths.
- Author:
Feifei LIANG
1
;
Yu YANG
;
Yimin ZHAO
;
Nengjiang YU
;
Jinhui WANG
Author Information
1. Institute of Pharmacology and Toxicology, Academy of Military Medical Science, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Angelica;
chemistry;
Chromatography, High Pressure Liquid;
methods;
Chromones;
analysis;
Coumarins;
analysis;
Drugs, Chinese Herbal;
analysis;
Furans;
analysis;
Furocoumarins;
analysis;
Rhizome;
chemistry;
Ultraviolet Rays
- From:
China Journal of Chinese Materia Medica
2010;35(19):2581-2584
- CountryChina
- Language:Chinese
-
Abstract:
A HPLC method was developed for simultaneouly quantitative analyses of aviprin (1), gosferol (2), 3'R-(+)-hamaudol (3), 3'-O-acetylhamaudol (4) and iso-imperatorin (5) in the rhizome of Angelica polymorpha. The analysis was performed at 25 degrees C on an Agilent Eclipse XDB-C18 analytical column (4.6 mm x 250 mm, 5 microm) with the mobile phase of methanol and H2O in gradient elution [55:45 (0 min)-65:35 (25 min)-95:5 (35 min)]. And the flow rate was 1.0 mL min(-1) with the detection wavelengths of 312 nm (1), 306 nm (2), 300 nm (3), 294 nm (4) and 310 nm (5). Consequently, the reqresion equations were Y = 1.81 x 10(3) X + 7.93 x 10(2) (r = 0.9996), Y = 2.49 x 10(3) X - 2.17 x 10(2) (r = 0.9993), Y = 2.02 x 10(3) X - 1.42 x 10(2) (r = 0.9991), Y = 1.57 x 10(3) X - 0.66 x 10(2) (r = 0.999 7), Y = 2.65 x 10(3) X - 1.47 x 10(2) (r = 0.999 6). And the average recoveries were 99.7% (RSD 0.57%), 100.1% (RSD 1.3%), 100.0% (RSD 1.6%), 99.6% (RSD 1.3%), 99.2% (RSD 0.59%), respectively. The precision, repeatability and stability were all consistent with the request of quantitative analysis. The contents of compound 1-5 in A. polymorpha were determined as 0.525%, 0.044%, 0.046%, 0.043%, 0.15%, respectively. Accordingly, this quantitative analysis method is good for the quality control of A. polymorpha.
