Metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide.
- Author:
Tongtong XU
1
;
Hui GUO
;
Bin WANG
;
Yuying ZHAO
;
Qingying ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Animals; Chromatography, High Pressure Liquid; Flavonoids; administration & dosage; metabolism; Magnetic Resonance Spectroscopy; Male; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2010;35(19):2585-2589
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide.
METHODGossypetin-8-O-beta-D-glucuronide was administrated orally at the dose of 250 mg x kg(-1) to healthy rats, then the urine was collected, pre-treated appropriately and detected by HPLC-DAD-MS(n).
RESULTIn the rat urine, gossypetin-8-O-beta-D-glucuronide, along with 6 metabolites, were detected, and their structures were elucidated on the basis of reference standards, UV and MS(n) spectra. The metabolic pathways of gossypetin-8-O-beta-D-glucuronide included mainly deglycosylation, glucuronidation and methylation.
CONCLUSIONA rapid and sensitive method for the analysis of metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide was established and this will be useful for the further metabolism study of gossypetin-8-O-beta-D-glucuronide.
