Study on effect of total saponins from Semen Nigellae on inflammatory mediators and ERK/MAPK pathway in stimulated macrophage.
- Author:
Dandan ZHANG
1
;
Xuqiang NIE
;
Huijun PAN
;
Linhua YU
;
Xiaolu YANG
;
Jinwen XU
;
Ka BIAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents; pharmacology; Cells, Cultured; Cyclooxygenase 2; metabolism; Humans; Inflammation; chemically induced; Inflammation Mediators; metabolism; Interleukin-6; metabolism; Lipopolysaccharides; pharmacology; Macrophages; drug effects; enzymology; metabolism; Mice; Nitric Oxide; metabolism; Nitric Oxide Synthase Type II; metabolism; Phosphorylation; drug effects; Saponins; pharmacology; Signal Transduction
- From: China Journal of Chinese Materia Medica 2010;35(19):2594-2598
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the anti-inflammatory mechanism of total saponins from Semen Nigellae (TSSN).
METHODIFN-gamma plus LPS stimulated RAW 264. 7 macrophage has been used as inflammatory experimental model. Griess reaction for nitric oxide production, FRAP assay for total antioxidant capacity, RT-PCR for mRNA expression and Western blot for protein expression examination were performed.
RESULTTSSN inhibited NO production in a dose-dependent manner. The gene and protein expression of iNOS were also suppressed by the herb extract. TSSN treatment significantly attenuated mRNA of inflammatory mediators such as COX-2, IL-1beta, IL-6 while increased PPAR-gamma gene and protein expression. Furthermore, phosphorylation of ERK (p-ERK) was markedly inhibited by TSSN.
CONCLUSIONTSSN suppressed pro-inflammatory mediators such as COX-2, IL-1beta, IL-6 and increased anti-inflammatory mediator PPAR-gamma expression. Meanwhile, TSSN inhibited over production of NO and iNOS expression through ERK/MAPK pathway.
