Determination of plasma concentration of N-methylcantharidimide by HPLC and its pharmacokinetics after intravenous administration in dogs.
- Author:
Xiaobo WU
1
;
Xianqin LUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Area Under Curve; Cantharidin; administration & dosage; analogs & derivatives; Chromatography, High Pressure Liquid; methods; Dogs; Female; Isoindoles; administration & dosage; blood; Male
- From: China Journal of Chinese Materia Medica 2010;35(19):2613-2615
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo establish a HPLC method for determination of N-methylcantharidimide in dogs' plasma and to study the pharmacokinetics of N-methylcantharidimide in dogs'.
METHODThe plasma samples were extracted by methanol. The acetonitrile and the purified water composed mobile phase. The flow rate was 0. 7 mL x min(-1), ultraviolet detection wavelength was at 212 nm.
RESULTThe calibration curve was linear over the range from 0.01-10.0 mg x L(-1) with a correlation coefficiency of 0.996 3. The lower limit of quantitation was 0.01 mg x L(-1). The mean recovery was 92.3%. the relative standard deviation (RSD) of intra-day and inter-day were all less than 10%. After intravenous administration of N-methylcantharidimide with 3 dosages of 10, 15, 20 mg x kg(-1) to dogs, the corresponding distribution half-livers (t1/2alpha) were 1.8, 2.1, 1.7 min, and the elimination half-lives (t1/2beta) were 144,139, 146 min, respectively.
CONCLUSIONThis method is convenient, accurate and reliable. It can be used for determination of N-methylcantharidimide in dogs' plasma and pharmacokinetic studies.
