Comparison of rutin and syringin content between tissue culturing seedlings and botanical drug of Saussurea involucrata.
- Author:
Yali LV
1
;
Chunlan WANG
;
Shunxing GUO
;
Hailing DONG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Glucosides; analysis; Phenylpropionates; analysis; Rutin; analysis; Saussurea; chemistry; growth & development; Seedlings; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(20):2666-2668
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the rutin and syringin content in tissue culturing seedlings and in botanical drug of Saussurea involucrata.
METHODThe HPLC with Hydro-RP C18 (4.6 mm x 250 mm, 5 microm) column was used, a mixture of acetonitrile-water (5:95) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 25 degrees C and detection wavelength at 220 nm.
RESULTThe effective constituents of tissue culturing seedlings were almost similar to the botanical drug. And syringin in tissue culturing seedlings was increased 4.35 times.
CONCLUSIONIt has a good prospect to acquire high-quality S. involucrata by tissue culturing seedlings.
