Optimization of extraction process of total amino acid from Bubali Cornu by orthogonal test.
- Author:
Qiang SHANG
1
;
Yan LIU
;
Jianxiong WU
;
Zhenzhong WANG
;
Lianming XU
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acids; isolation & purification; pharmacology; Animals; Buffaloes
- From: China Journal of Chinese Materia Medica 2010;35(20):2693-2695
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the extraction conditions of the total amino acid from Bubali Cornu.
METHODAn orthogonal test of L9 (3(4)) was designed to select optimum exaction conditions of the total amino acid. The influence of solvent concentration, the dosage of solvent and time of extraction were investigated with the content of total amino acid and thrombin-induced fibrin clotting time as index.
RESULTThe optimum extraction condition procedure was described as follows: the concentration of the solvent was 4 mol x L(-1), volume of solvent was 6 times amount of the materials.
CONCLUSIONThe optimum exaction conditions procedure is reasonable and stable.
