Simultaneous determination of rutin, isoquercitrin and chlorogenic acid in Farfarae Flos by HPLC.
- Author:
Di WU
1
;
Zhengtao WANG
;
Mian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Calibration; Chlorogenic Acid; analysis; Chromatography, High Pressure Liquid; methods; Quercetin; analogs & derivatives; analysis; Rutin; analysis; Tussilago; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(20):2722-2725
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for simultaneous determination of rutin, isoquercitrin and chlorogenic acid in Farfarae Flos.
METHODThe analysis was carried out on a Phenomenex Synergi POLAR-RP 80A column (4.6 mm x 250 mm, 4 microm) with gradient elution using methanol-acetonitrile-water (adjusted to pH 2.5 with formic acid) as mobile phase. The flow rate was 1.2 mL x min(-1) and the detection wavelength was at 255 nm.
RESULTThe calibration curves were linear over the range of 0.2-2 000 microg x L(-1) for rutin and isoquercitrin, 10-2 000 microg x L(-1) for chlorogenic acid, respectively. The average recoveries were 99.5% for rutin, 100.1% for isoquercitrin and 99.4% for chlorogenic acid, respectively, with RSD not more than 3.0%.
CONCLUSIONThe described method is reliable and could be used for the quality control of Farfarae Flos.
