Study of immunogenicity of chlorogenic acid-BSA with different number of CGA.
- Author:
Liyan YUAN
1
;
Ping LIU
;
Yuan HU
;
Hongbo LIAO
;
Lihua MU
;
Hui LIN
;
Honglin HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chlorogenic Acid; immunology; Enzyme-Linked Immunosorbent Assay; Histamine; blood; Immunoglobulin E; blood; Mice; Mice, Inbred BALB C; Passive Cutaneous Anaphylaxis; Rats; Rats, Sprague-Dawley; Serum Albumin, Bovine; immunology
- From: China Journal of Chinese Materia Medica 2010;35(20):2756-2759
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the immunogenicity of chlorogenic acid-BSA(CGA-BSA) and the foundation for the allergization of CGA-BSA.
METHODThe CGA-BSA with different number of CGA was synthesized to allergize the BALB/c mice and SD rats to get antiserum. The level of IgE and histamine was analyzed by ELISA, and the passive cutaneous anaphylaxis (PCA) test was carried to analyze the antibody titer.
RESULTWhen the coupling rate of CGA-BSA is 20, the highest IgE and histamine level in BALB/c mice and the antibody titer in PCA are the highest.
CONCLUSIONIt has better immunogenicity when the coupling rate of CGA-BSA is between 10 to 20, while the CGA-BSA 20 with the best immunogenicity.
