Effect of assemble flavone of Drynariae Rhizome on activity and calcium deposition of bone marrow stem cells of rabbit.
- Author:
Xiaochun SHU
1
;
Xiangyang HU
;
Lijuan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Animals; Bone Marrow Cells; cytology; drug effects; metabolism; Calcium; metabolism; Cell Differentiation; drug effects; Female; Flavones; pharmacology; Male; Mesenchymal Stromal Cells; cytology; drug effects; metabolism; Polypodiaceae; chemistry; Rabbits
- From: China Journal of Chinese Materia Medica 2010;35(20):2760-2763
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of assemble flavone of Drynariae Rhizome on the activity and osteogenic differentiation of bone marrow stem cells of rabbit in vitro.
METHODDensity gradient centrifugation was used to isolate bone marrow stem cells from SD rats. The MSCs were also undergone osteogenic induction. Cytochemical and immunocytochemical tainting were performed to verify their multipotential.
RESULTHigh purity of bone marrow stem cells was successfully obtained. Both ALP activity and mineralization nodes tainting were positive in MSCs after osteogenic differentiation.
CONCLUSIONThe MSCs sustained their osteogenic differentiation potential in vitro by assemble flavone of rhizome drynaria.
