Study on HPLC fingerprinting and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice.
- Author:
Dongan CHEN
1
;
Jinhai YI
;
Zhifang HUANG
;
Yunhua LIU
;
Yan WU
;
Xiaoliang LI
Author Information
- Publication Type:Journal Article
- MeSH: Aconitum; chemistry; Alkaloids; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; standards; Medicine, Chinese Traditional; standards; Quality Control
- From: China Journal of Chinese Materia Medica 2010;35(21):2829-2833
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the HPLC fingerprint and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice.
METHODHPLC analysis was performed on a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) with 0.04 mol x L(-1) ammonium acetate (adjusted to pH 10.0 with ammonia water)and acetonitrile as mobile phase. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004 AB)was used in data analysis.
RESULTBaifupian, Heifupian from different samples were of high similarity in fingerprint, and the separation of six kinds of ester-type alkaloids was good. Huangfupian and crude aconite root showed significant difference in fingerprint, comparing with Baifupian and Heifupian.
CONCLUSIONThis method is simple and reliable. The HPLC fingerprint and contents of six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice can be used for their quality control.
