HPLC fingerprints of processed products from Sinapis alba.
- Author:
Cun ZHANG
1
;
Li LI
;
Yongqing XIAO
;
Zhen PANG
;
Guiliu LI
Author Information
- Publication Type:Journal Article
- MeSH: Chemistry, Pharmaceutical; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Sinapis; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(21):2842-2845
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the HPLC fingerprint method and compare the changes of chemical compositions of the processed products from Sinapis alba.
METHODThe procedure of HPLC analysis was performed on a agilent TC-C18 (2) column at 35 degrees C with the acetonitrile -0.1% phosphoric acid in gradient elution as the mobile phase. The detection wavelength was set at 254 nm, and the flow rate was 1.0 mL x min(-1). The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (Version 2004 A) was applied to analyze the similarity.
RESULTThe average similarities among the processed products were over 0.96, the standard HPLC fingerprint and five main chromatographic peaks with the isolated compounds was obtained and identified.
CONCLUSIONThe established methods with two solvents are suitable for the HPLC fingerprints determination which elementary elucidate the scientific intensions of breaking the enzyme for glycosides.
