Effects of HSYA on expression of bFGF protein and MMP-9 in BGC-823 transplantation tumor of nude mice.
- Author:
Shengyan XI
1
;
Qian ZHANG
;
Chaoyang LIU
;
Hua XIE
;
Lifeng YUE
;
Yufang ZHAO
;
Baoxia ZANG
;
Xuemin GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Disease Models, Animal; Drugs, Chinese Herbal; administration & dosage; Female; Fibroblast Growth Factor 2; genetics; metabolism; Gene Expression Regulation, Neoplastic; drug effects; Humans; Matrix Metalloproteinase 9; genetics; metabolism; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Neovascularization, Pathologic; Stomach Neoplasms; drug therapy; genetics; metabolism; pathology
- From: China Journal of Chinese Materia Medica 2010;35(21):2877-2881
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of hydroxy safflor yellow A (HSYA) on the expression of bFGF protein and MMP-9 mRNA or protein of transplantation tumor of gastric adenocarcinoma cell line BGC-823 in nude mice.
METHODThe BGC-823 cells were subcutaneously injected into the right anterior armpit of BALB/C nu/nu nude mice, and the animal model of transplantation tumor was established. The experimental groups were treated with HSYA at concentration of 0.056 and 0.028 g x L(-1) and cyclophosphamide at 2 g x L(-1), or with physiologic saline. The tumor inhibitory effect was observed, and the mRNA expression of MMP-9 of transplantation tumor was detected by real time-fluorescent quantitation PCR and the protein expression of MMP-9 and bFGF were detected by enzyme linked immunosorbent assay.
RESULTThe IR in the group with HSYA at the concentration of 0.028 g x L(-1) is higher than in the group with normal sodium. After treatment with HSYA, the mRNA expression of MMP-9 has significant difference at the concentration of 0.028 g x L(-1) as compared with physiologic saline-treated group (P < 0.05), but the protein expression of MMP-9 and bFGF is obviously less than that in the physiologic saline-treated group (P < 0.05).
CONCLUSIONThe possible mechanism of HSYA in given concentration to antagonize tumor angiogenesis may be related with inhibiting the protein expression of MMP-9 and bFGF or the mRNA expression of MMP-9 in tumor tissue to reduce the degradation of blood vessel basilar membrane, and to restrain the migration of blood vessel and decrease the tumor vascularization.