The activation of vagus afferent in response to lipopolysaccharide the role of interleukin-1.
- Author:
Xiu-Ying LU
1
;
Gui-Zhen YANG
;
Hui-Chen SUN
Author Information
1. Norman Bethune National Peace Hospital, Shijiazhuang 050082. xiuyinglu@joymail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Cells, Cultured;
Interleukin-1;
biosynthesis;
physiology;
Lipopolysaccharides;
pharmacology;
Macrophages;
drug effects;
metabolism;
Male;
Neurons, Afferent;
drug effects;
metabolism;
Proto-Oncogene Proteins c-fos;
biosynthesis;
Rats;
Rats, Wistar;
Vagus Nerve;
drug effects;
metabolism
- From:
Acta Physiologica Sinica
2002;54(2):111-114
- CountryChina
- Language:Chinese
-
Abstract:
To study the possibility of activation of vagus afferent in response to lipopolysaccharide (LPS) through interleukin-1 (IL-1), Wistar rats were randomly divided into LPS group and saline (NS) group. The expression of c-Fos, CD14 and Mac-1 were detected by immunohistochemistry staining. IL-1 bioactivity was determined by L929 cell proliferation. The expression of IL-1R I mRNA was detected by in situ hybridization. Our results showed that there were some c-Fos protein expression positive neurons in the nodose ganglion in LPS group, but no c-Fos protein expression positive neurons in the nodose ganglion in control group. The number of macrophages (M phi) in the connective tissue surrounding the abdominal vagus increased significantly in response to LPS i.p. Forty-five minutes after the application of LPS, the IL-1 bioactivity in the supernatant of M phi was increased. Positive IL-1R mRNA neurons were also observed in the nodose ganglion in the LPS group. The results indicate that vagus afferent is activated in response to LPS and that IL-1 production might be involved in the activation of vagus afferent.
