Relationship between proliferation of vascular smooth muscle cells and PDGF-AA and PDGFR-alpha expression in SHRs.
- Author:
Jun JIN
1
;
Shan-Jun ZHU
;
Zhi-Ming ZHU
;
Yong-Jian YANG
;
Gang DING
Author Information
1. Department of Cardiology, Xingqiao Hospital, Third Military Medical University, Chongqing 400037.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Division;
drug effects;
Cells, Cultured;
Hypertension;
physiopathology;
Male;
Muscle, Smooth, Vascular;
cytology;
drug effects;
metabolism;
Platelet-Derived Growth Factor;
biosynthesis;
pharmacology;
Rats;
Rats, Inbred SHR;
Rats, Inbred WKY;
Receptor, Platelet-Derived Growth Factor alpha;
biosynthesis
- From:
Acta Physiologica Sinica
2002;54(2):145-148
- CountryChina
- Language:Chinese
-
Abstract:
To explore the role of platelet derived growth factor-AA (PDGF-AA) and PDGFR-alpha expression in the proliferation and hypertrophy of vascular smooth muscle cells (VSMCs) in spontaneously hypertension rats (SHR), protein expression of PDGF-AA, PDGFR-alpha and PDGFR-beta in SHR/Wistar-Kyoto (WKY)-VSMC was observed by Western blot. Proliferation and hypertrophy of SHR-VSMCs induced by PDGF-AA were observed by measurement of PCNA and [(3)H] incorporation. PDGF-AA and PDGFR-alpha expression was markedly increased in SHR-VSMCs compared with that in WKY-VSMCs, but PDGFR-beta was not different in SHR and WKY-VSMCs. PDGF-AA induced PCNA expression and [(3)H] incorporation was increased in a dose-dependent manner in SHR, but not in WKY. It is suggested that an enhancement of PDGF-AA and PDGFR-alpha in SHRs may be one of the important factors for vascular modeling.
