Intracellular dialysis with a microcatheter inserted into the patch-clamp pipette.
- Author:
Guo-Hua LI
1
;
Zhi-Wang LI
;
Shi-Duan WANG
;
Jin-Bo WEI
;
Xian-Ke ZHENG
Author Information
1. Department of Physiology, Yunyang Medical College, Shiyan 442000.
- Publication Type:Journal Article
- MeSH:
Dialysis;
instrumentation;
methods;
Equipment Design;
Microelectrodes;
Patch-Clamp Techniques;
instrumentation
- From:
Acta Physiologica Sinica
2002;54(2):179-182
- CountryChina
- Language:Chinese
-
Abstract:
In this paper we present an easily available method of intracellular dialysis via a microcatheter inserted into glass pipette during patch clamp experiment. An oblique hole through the glass pipette holder (above the lateral hole for cell-seal suction) is drilled, through which a microcatheter (O.D.=0.1 mm) made from the universal pipetter tip by hand-drawing passes and sticks out of the holder mouth in parallel with the Ag-AgCl electrode. With a syringe connected to the microcatheter, substitution of intracellular solution and intracellular dialysis of drugs can be achieved easily. Compared with repatch technique and intracellular solution substitution techniques used abroad, this method operates more easily and can produce more reliable results.
